Eragrostis tef Assembly and Gene Annotation

About Eragrostis tef

Eragrostis tef, known as tef or teff, is a small-seeded allotetraploid cereal (2C=2n=4x=40). It is grown as a crop for human consumption in the Horn of Africa and cultivated for hay in countries such as South Africa, Australia, and the USA.

Assembly

A chromosome-scale assembly of the allotetraploid teff genome was built using a combination of long read single-molecule real-time sequencing and long-range high-throughput chromatin capture (Hi-C).

Annotation

The teff genome was annotated using the MAKER pipeline. Transcript support from a large-scale expression atlas and protein homology to Arabidopsis and other grass genomes were used as evidence for ab initio gene prediction. After filtering transposon-derived sequences, ab initio gene prediction identified 68,255 gene models.

References

• Exceptional subgenome stability and functional divergence in the allotetraploid Ethiopian cereal teff.
  VanBuren R, Man Wai C, Wang X, Pardo J, Yocca AE, Wang H, Chaluvadi SR, Han G, Bryant D, Edger PP, Messing J, Sorrells ME, Mockler TC, Bennetzen JL, Michael TP.. Nat Commun 11 (1)

Picture credit: Kaye Murri tef cultivar, Gina Cannarozzi

Links

• www.tef-research.org/genome.html
• http://130.92.252.158/tef/version1