Glycine max (Glycine_max_v2.1)

Glycine max Assembly and Gene Annotation

About Glycine max

Glycine max (soybean) is a crop legume that globally constitutes one of the most important sources of animal feed protein and cooking oil. Having originated in East Asia soy is now cultivated world-wide with greatest production in the U.S. Though only a minor proportion of the crop is eaten directly by humans, soybean is a valuable source of protein, containing all essential amino acids, and frequently used as a dietary substitute for meat. Like other legumes, soybean is able to fix atmospheric nitrogen by engaging in a symbiotic relationship with microbial organisms. The complete sequence of the soybean genome not only impacts research and breeding of this crop, but also serves as a reference for genomics research in other legumes. Representing the order Fabales within the eudicot taxonomy, the sequence will also advance research in comparative phylogenomics. As a paleopolyploid, the soybean genome shows evidence of two ancient whole genome duplications, one early in the legume lineage and a second more recent event specific to the soybean lineage. The soybean genome has 20 chromosomes and an estimated size of 1,115 Mb.


Glycine max var. Williams 82 was sequenced, assembled, and annotated by the U.S. DOE Joint Genome Institute (JGI-PGF) in collaboration with a consortium of research labs and published. Current assembly is version v2.1 and comprises a total 978 Mb (GCA_000004515.4{.external-link}).


The Wm82.a2.v1 gene set integrates ~1.6 million ESTs, some 454 ESTs and 1.5 billion paired-end Illumina RNA-seq reads with homology-based gene predictions. A total of 56,044 protein-coding loci (see JGI Phytozome for additional details).


Variation data from the European Variation Archive was added.

The data is from a project conducted to investigate selection signatures in soybean subgroups. 112 Glycine soja and 133 Glycine max accessions were re-sequenced, and numerous variants for the 245 accessions were obtained through variant calling process using GATK. Then, to avoid as false-positive variants as much possible, variant-quality-filtering and allele-frequency-filtering processes were conducted to the obtained variants. Finally, 9,650,073 bi-allelic SNP variants with minor allele frequency > 1% were identified for the 245 soybean accessions and utilized to this project [3].


  1. Genome sequence of the palaeopolyploid soybean.
    Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J et al. 2010. Nature. 463:178-183.
  2. Image credit: USDA.
  3. Dissection of soybean populations according to selection signatures based on whole-genome sequences.
    Jae-Yoon Kim, Seongmun Jeong, Kyoung Hyoun Kim, Won-Jun Lim, Ho-Yeon Lee, Namhee Jeong, Jung-Kyung Moon, Namshin Kim. GigaScience, Volume 8, Issue 12, 2019.

Picture credit: Wikimedia Commons, the free media repository

More information

General information about this species can be found in Wikipedia.



AssemblyGlycine_max_v2.1, INSDC Assembly GCA_000004515.4, Jul 2018
Database version104.4
Golden Path Length978,491,270
Genebuild byJGI
Genebuild methodImport
Data sourceJoint Genome Institute

Gene counts

Coding genes55,897
Gene transcripts88,412

About this species