Chlamydomonas reinhardtii Assembly and Gene Annotation
About Chlamydomonas reinhardtii
Chlamydomonas reinhardtii is a unicellular green alga from the phylum Chlorophyta, which diverged from land plants over a billion years ago. C. reinhardtii is a model species for studying a broad range of fundamental biological processes including the evolution of chloroplast-based photosynthesis and the structure of eukaryotic flagella. It is haploid, and has a nuclear genome comprising 17 chromosomes with a total size of ~120 Mb.
The 121 Mb draft sequence of the Chlamydomonas nuclear genome was generated at 13x coverage by whole-genome, shotgun sequencing of plasmid and fosmid libraries, followed by assembly into ~1500 contigs by JGI. Assembly into chromosomes was done on the basis of genetic mapping.
The set of gene predictions in version 5.5 incorporates JGI gene expression data from 1 million reads generated from nitrogen-starved cells on the 454 Titanium platform as well as 239 million 2x150 stranded Illumina reads and other smaller RNA-seq datasets. The underlying gene prediction was performed by Mario Stanke using Augustus (annotation version u11.6).
- The Chlamydomonas genome reveals the evolution of key animal and
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Marchal-Drouard L et al. 2007. Science. 318:245-250.
Picture credit: By Ninghui Shi [CC BY-SA 3.0 (https://creativecommons.org/licenses/by-sa/3.0)], from Wikimedia Commons
General information about this species can be found in Wikipedia.
|Assembly||Chlamydomonas_reinhardtii_v5.5, INSDC Assembly GCA_000002595.3, Jan 2018|
|Golden Path Length||111,098,438|
|Data source||Joint Genome Institute|
|Non coding genes||200|
|Small non coding genes||200|