Oryza sativa Japonica Group vs Trifolium pratense LastZ Results

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Oryza sativa Japonica Group (Oryza sativa Japonica Group, IRGSP-1.0) and Trifolium pratense (Trifolium pratense, Trpr) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Plants release . Oryza sativa Japonica Group was used as the reference species. After running LastZ, the raw LastZ alignment blocks are chained according to their location in both genomes. During the final netting process, the best sub-chain is chosen in each region on the reference species.

Results

Number of alignment blocks: 140,564

Genome coverage (bp) Coding exon coverage (bp)
Oryza sativa Japonica Group

Uncovered: 343,357,036 out of 375,049,285
Covered: 31,692,249 out of 375,049,285

Uncovered: 16,193,909 out of 34,403,043
Matches: 11,422,129 out of 34,403,043
Mismatches: 6,407,801 out of 34,403,043
Insertions: 379,204 out of 34,403,043

Trifolium pratense

Uncovered: 272,193,050 out of 304,842,038
Covered: 32,648,988 out of 304,842,038

Uncovered: 20,205,561 out of 41,242,947
Matches: 13,496,534 out of 41,242,947
Mismatches: 7,121,210 out of 41,242,947
Insertions: 419,642 out of 41,242,947

Configuration parameters

ParameterValue
Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91
Other parameters (other)--ambiguous=iupac

Chunking parameters

Oryza sativa Japonica Group Trifolium pratense
Chunk size 10,000,000 10,100,000
Overlap 0 100,000
Group set size 0 10,100,000
Masking options {default_soft_masking => 1} {default_soft_masking => 1}