Arabidopsis thaliana vs Hordeum vulgare LastZ results
Arabidopsis thaliana (Arabidopsis thaliana, TAIR10) and Hordeum vulgare (Hordeum vulgare subsp. vulgare, IBSC_v2) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Plants release 94. Arabidopsis thaliana was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
Parameter | Value |
---|---|
Gap open penalty (O) | 400 |
Gap extend penalty (E) | 30 |
HSP threshold (K) | |
Threshold for gapped extension (L) | 3000 |
Threshold for alignments between gapped alignment blocks (H) | 2200 |
Masking count (M) | |
Seed and Transition value (T) | 1 |
Scoring matrix (Q) | Default: A C G T 91 -114 -31 -123 -114 100 -125 -31 -31 -125 100 -114 -123 -31 -114 91 |
Chunking parameters
Parameter | Arabidopsis thaliana | Hordeum vulgare |
---|---|---|
Chunk size | 10,000,000 | 10,100,000 |
Overlap | 0 | 100,000 |
Group set size | 0 | 10,100,000 |
Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 165,915 alignment blocks
Genome coverage (bp) | Coding exon coverage (bp) | |
---|---|---|
Arabidopsis thaliana |
Uncovered: 92,861,049 out of 119,667,750 |
Uncovered: 14,177,475 out of 33,775,569 |
Hordeum vulgare |
Uncovered: 4,788,489,275 out of 4,834,432,680 |
Uncovered: 21,702,856 out of 44,420,163 |
Block size distribution
Size range | All 165,915 alignment blocks | Blocks grouped in nets | |||
---|---|---|---|---|---|
# blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
1 bp - 10 bp | 932 |
4.9 kb |
|||
10 bp - 100 bp | 26,068 |
1.8 Mb |
7,054 |
522.7 kb |
|
100 bp - 1 kb | 129,577 |
42.5 Mb |
50,302 |
19.7 Mb |
|
1 kb - 10 kb | 9,338 |
14.1 Mb |
19,403 |
37.7 Mb |
|
10 kb - 100 kb | 26 |
383.2 kb |