Oryza meridionalis vs Oryza sativa Japonica Group LastZ results

Oryza meridionalis (Oryza meridionalis, Oryza_meridionalis_v1.3) and Oryza sativa Japonica Group (Oryza sativa Japonica Group, IRGSP-1.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Plants release 79. Oryza meridionalis was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.

Configuration parameters

Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)3000
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91

Chunking parameters

ParameterOryza meridionalisOryza sativa Japonica Group
Chunk size30,000,00010,100,000
Group set size010,100,000
Masking options{default_soft_masking => 1}

Statistics over 343,106 alignment blocks

Genome coverage (bp) Coding exon coverage (bp)
Oryza meridionalis

Uncovered: 97,874,978 out of 335,668,232
Covered: 237,793,254 out of 335,668,232

Uncovered: 420,569 out of 35,224,824
Matches: 33,566,367 out of 35,224,824
Mismatches: 995,076 out of 35,224,824
Insertions: 242,812 out of 35,224,824
Identity over aligned base-pairs: 96.4%

Oryza sativa Japonica Group

Uncovered: 183,181,862 out of 374,424,240
Covered: 191,242,378 out of 374,424,240

Uncovered: 5,949,666 out of 34,124,807
Matches: 27,452,391 out of 34,124,807
Mismatches: 572,333 out of 34,124,807
Insertions: 150,417 out of 34,124,807
Identity over aligned base-pairs: 97.4%