Triticum dicoccoides vs Triticum turgidum LastZ results
Triticum dicoccoides (Triticum dicoccoides, WEWSeq_v.1.0) and Triticum turgidum (Triticum turgidum subsp. durum, Svevo.v1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Plants release 100. Triticum dicoccoides was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value | 
|---|---|
| Gap open penalty (O) | 400 | 
| Gap extend penalty (E) | 30 | 
| HSP threshold (K) | 5000 | 
| Threshold for gapped extension (L) | 5000 | 
| Threshold for alignments between gapped alignment blocks (H) | 3000 | 
| Masking count (M) | 10 | 
| Seed and Transition value (T) | 1 | 
| Scoring matrix (Q) | Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91
 | 
Chunking parameters
| Parameter | Triticum dicoccoides | Triticum turgidum | 
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 | 
| Overlap | 0 | 100,000 | 
| Group set size | 0 | 10,100,000 | 
| Masking options | 
Statistics over 1,996,754 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Triticum dicoccoides | 
                                              Uncovered: 2,243,046,094 out of 10,079,039,394 | 
            Uncovered: 13,105,125 out of 81,268,905 | 
| Triticum turgidum | 
                                              Uncovered: 2,415,799,529 out of 10,463,058,104 | 
            Uncovered: 16,093,563 out of 88,577,667 | 
Block size distribution
| Size range | All 1,996,754 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 25,332 | 143.7 kb | |||
| 10 bp - 100 bp | 402,045 | 21.7 Mb | 179,952 | 10.5 Mb | |
| 100 bp - 1 kb | 813,165 | 312.0 Mb | 395,257 | 144.3 Mb | |
| 1 kb - 10 kb | 524,939 | 1.8 Gb | 204,761 | 634.5 Mb | |
| 10 kb - 100 kb | 229,029 | 6.0 Gb | 12,799 | 207.5 Mb | |
| 100 kb - 1 Mb | 2,244 | 276.6 Mb | 788 | 301.1 Mb | |
| 1 Mb - 10 Mb | 161 | 522.1 Mb | |||
| 10 Mb - 100 Mb | 29 | 480.0 Mb | |||
| 100 Mb - 1 Gb | 14 | 6.2 Gb | |||


 
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