Arabidopsis thaliana vs Chondrus crispus LastZ results
Arabidopsis thaliana (Arabidopsis thaliana, TAIR10) and Chondrus crispus (Chondrus crispus, ASM35022v2) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Plants release 94. Arabidopsis thaliana was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value | 
|---|---|
| Gap open penalty (O) | 400 | 
| Gap extend penalty (E) | 30 | 
| HSP threshold (K) | |
| Threshold for gapped extension (L) | 3000 | 
| Threshold for alignments between gapped alignment blocks (H) | 2200 | 
| Masking count (M) | |
| Seed and Transition value (T) | 1 | 
| Scoring matrix (Q) | Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91
 | 
Chunking parameters
| Parameter | Arabidopsis thaliana | Chondrus crispus | 
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 | 
| Overlap | 0 | 100,000 | 
| Group set size | 0 | 10,100,000 | 
| Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} | 
Statistics over 7,927 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Arabidopsis thaliana | 
                                              Uncovered: 118,049,190 out of 119,667,750 | 
            Uncovered: 32,274,028 out of 33,775,569 | 
| Chondrus crispus | 
                                              Uncovered: 104,028,320 out of 104,980,420 | 
            Uncovered: 9,419,718 out of 10,311,317 | 
Block size distribution
| Size range | All 7,927 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 25 | 147.0 bp | |||
| 10 bp - 100 bp | 2,163 | 158.1 kb | 242 | 18.3 kb | |
| 100 bp - 1 kb | 5,556 | 1.4 Mb | 2,210 | 949.9 kb | |
| 1 kb - 10 kb | 183 | 252.2 kb | 532 | 820.8 kb | |


 
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