Oryza meridionalis vs Oryza sativa Japonica Group LastZ results
Oryza meridionalis (Oryza meridionalis, Oryza_meridionalis_v1.3) and Oryza sativa Japonica Group (Oryza sativa Japonica Group, IRGSP-1.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Plants release 79. Oryza meridionalis was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Oryza meridionalis | Oryza sativa Japonica Group |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options | {default_soft_masking => 1} |
Statistics over 343,106 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Oryza meridionalis |
Uncovered: 97,874,978 out of 335,668,232 |
Uncovered: 420,569 out of 35,224,824 |
| Oryza sativa Japonica Group |
Uncovered: 183,806,907 out of 375,049,285 |
Uncovered: 6,100,995 out of 34,818,902 |
Block size distribution
| Size range | All 343,106 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 4,170 |
23.8 kb |
|||
| 10 bp - 100 bp | 76,724 |
4.6 Mb |
37,948 |
2.4 Mb |
|
| 100 bp - 1 kb | 202,043 |
69.7 Mb |
88,437 |
28.3 Mb |
|
| 1 kb - 10 kb | 59,055 |
154.4 Mb |
13,787 |
30.1 Mb |
|
| 10 kb - 100 kb | 1,114 |
14.8 Mb |
508 |
12.5 Mb |
|
| 100 kb - 1 Mb | 53 |
10.5 Mb |
|||
| 1 Mb - 10 Mb | 3 |
24.1 Mb |
|||
| 10 Mb - 100 Mb | 9 |
135.8 Mb |
|||


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