Medicago truncatula vs Brassica napus LastZ results
Medicago truncatula (Medicago truncatula, MedtrA17_4.0) and Brassica napus (Brassica napus, AST_PRJEB5043_v1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Plants release 94. Medicago truncatula was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
Parameter | Value |
---|---|
Gap open penalty (O) | 400 |
Gap extend penalty (E) | 30 |
HSP threshold (K) | |
Threshold for gapped extension (L) | 3000 |
Threshold for alignments between gapped alignment blocks (H) | 2200 |
Masking count (M) | |
Seed and Transition value (T) | 1 |
Scoring matrix (Q) | Default: A C G T 91 -114 -31 -123 -114 100 -125 -31 -31 -125 100 -114 -123 -31 -114 91 |
Chunking parameters
Parameter | Medicago truncatula | Brassica napus |
---|---|---|
Chunk size | 10,000,000 | 10,100,000 |
Overlap | 0 | 100,000 |
Group set size | 0 | 10,100,000 |
Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 403,390 alignment blocks
Genome coverage (bp) | Coding exon coverage (bp) | |
---|---|---|
Medicago truncatula |
Uncovered: 355,594,792 out of 412,800,391 |
Uncovered: 19,210,398 out of 50,132,764 |
Brassica napus |
Uncovered: 730,521,989 out of 848,200,303 |
Uncovered: 30,305,197 out of 101,157,402 |
Block size distribution
Size range | All 403,390 alignment blocks | Blocks grouped in nets | |||
---|---|---|---|---|---|
# blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
1 bp - 10 bp | 3,704 |
20.2 kb |
|||
10 bp - 100 bp | 63,575 |
4.2 Mb |
15,806 |
1.1 Mb |
|
100 bp - 1 kb | 311,580 |
104.1 Mb |
90,521 |
35.3 Mb |
|
1 kb - 10 kb | 24,282 |
38.7 Mb |
48,164 |
106.6 Mb |
|
10 kb - 100 kb | 248 |
6.9 Mb |
444 |
10.1 Mb |
|
100 kb - 1 Mb | 1 |
100.1 kb |
8 |
1.0 Mb |